11/7/2023 0 Comments Indeterminate western blot![]() Results highlight the need for further research to provide reliable estimates of the extensiveness of indeterminate HIV test results and to further assess the nature of counselor and client responses to indeterminate test results. Counselors reported that communicating indeterminate test results to clients was a challenging task because clients who received such a result were confused, angry, and/or mistrustful. Counselors' primary advice to clients who received an indeterminate result was to retest immediately without a waiting period. Applying the Western Blot (WB) technique, screening for HTLV I/II in blood donors has shown incomplete antibody reactivity against viral antigens, which has. Some counselors believed that an indeterminate result truly was ambiguous while others believed it was indicative of being either HIV-positive or HIV-negative. Counselors' understanding of the meaning of an indeterminate HIV test result varied a great deal. Non-B subtypes are detected by current HIV-1 Western blots, with sensitivity and specificity equal to those for subtype B. They also presented several different theories of causation, yet failed to mention two potential causes of an indeterminate result, association with large animals and infection with other (non-HIV-1) HIV strains. An indeterminate Western blot result should prompt repeat testing with Western blot in 2-4 weeks unless acute HIV-1 or HIV-2 infection is suspected. I/II Seropositive, Seronegative and Indeterminate. ting of a positive enzyme immunoassay (EIA) and Western blot (WB) was extremely low 3, 4. Note: Negative dried blood spot specimens frequently. Counselors defined an indeterminate test result in different ways. MAK 0018-ENG-4 Revision Date: 12/14 (18 tests kit): 0711088018 (36 tests. Interpret the immunoblot as NEGATIVE, INDETERMINATE, or POSITIVE based on the pattern that is present. Distinguishing between HTLV-I and HTLV-II by Western blot. Field observations of three community HIV testing sites and semistructured interviews with 16 HIV test counselors were conducted from August 2002 through June 2003. Low incidence of HTLV infections in random blood donors with indeterminate Western blot patterns. There are many reasons for an indeterminate HIV antibody/ Western blot assay including early HIV infection, infection with other contagious diseases, autoimmune diseases, and second or subsequent pregnancies in women. HTLV-II sequences were detected by PCR in one of the Western blot-positive samples, as well as in one Western blot-indeterminate sample that showed reactivity to p24 only. The detection of p24 antigen and plasma RNA can also be used to confirm HIV-1 infection in persons with indeterminate serologic results associated with early seroconversion or late-stage disease.This study examined HIV test counselors' beliefs and practices regarding communicating indeterminate HIV test results to clients. Of the 61 repeatably reactive samples, 2 were positive, 26 were negative, and 33 were interpreted as indeterminate on Western blot. The detection of plasma RNA appears to be sensitive and specific negative test results may be used to identify false-positive serologic reactions. One of 72 blood donor specimens was positive for HIV-1 plasma RNA and had borderline reactivity for p24 antigen. All of 57 diagnostic specimens and 47 samples obtained from persistently indeterminate donors were negative for HIV-1 p24 antigen and plasma HIV-1 RNA. HIV-1 plasma RNA was positive in 22 of 22 samples from those 14 individuals. HIV-1 p24 antigen was detected in 10 of 20 specimens collected from 9 of 12 individuals who seroconverted and in 2 of 2 AIDS patients. Background: Most enzyme immunoassay-reactive specimens producing indeterminate Western blot results belong to individuals who are not infected with human immunodeficiency virus type 1 (HIV-1). HTLV-I/II infection in a high viral endemic area of Zaire, Central Africa: comparative evaluation of serology, PCR, and significance of indeterminate Western-blot. Samples were obtained from infected individuals at the time of seroconversion (n = 20), from patients with AIDS (n = 2), as specimens from clinical samples obtained for diagnostic testing (n = 57), from blood donors producing persistent indeterminate results (n = 47), and from random blood donors (n = 72). To determine the utility of HIV-1 p24 antigen and cell-free RNA detection for the resolution of Western blot-indeterminate serologic results, several types of enzyme immunoassay-positive, sero-indeterminate specimens were analyzed. However, a small percentage may correspond to early seroconversion or advanced disease, at which stage partial reactivity on Western blot may be observed. ![]() Most enzyme immunoassay-reactive specimens producing indeterminate Western blot results belong to individuals who are not infected with human immunodeficiency virus type 1 (HIV-1).
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